length mat seca 211 Search Results


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Seca 210 infantometer measuring mat
210 Infantometer Measuring Mat, supplied by Seca, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Seca homologues
Homologues, supplied by Seca, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Seca seca translation cis regulator secm
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secm  (Seca)
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Seca secm
Secm, supplied by Seca, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Seca protein translocation
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Seca extrato seco massa da amostra vegetal seca × 100 figura 2
Extrato Seco Massa Da Amostra Vegetal Seca × 100 Figura 2, supplied by Seca, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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secg  (Seca)
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Seca secg
Secg, supplied by Seca, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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height  (Seca)
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Seca height
Height, supplied by Seca, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Seca body mass
Body Mass, supplied by Seca, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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seca2  (Seca)
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Seca seca2
FIGURE 1. Accessory sec locus of S. gordonii DL1. Schematic representation of the 20.5-kb accessory sec locus (derived from the genome sequence of S. gordonii CH1: GenBankTM accession number CP000725.1). Surface protein, hsa (SGO_0966); glycosyltransferases, gly (SGO_0968), nss (SGO_0969), gtfA (SGO_0975), and gtfB (SGO_0976); accessory secretion proteins, asp1 (SGO_0971), asp2 (SGO_0972), asp3 (SGO_0973), asp4 (SGO_0977), and asp5 (SGO_0978); secY2 (SGO_0970), <t>secA2</t> (SGO_0974).
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Seca translocation machinery
FIGURE 1. Accessory sec locus of S. gordonii DL1. Schematic representation of the 20.5-kb accessory sec locus (derived from the genome sequence of S. gordonii CH1: GenBankTM accession number CP000725.1). Surface protein, hsa (SGO_0966); glycosyltransferases, gly (SGO_0968), nss (SGO_0969), gtfA (SGO_0975), and gtfB (SGO_0976); accessory secretion proteins, asp1 (SGO_0971), asp2 (SGO_0972), asp3 (SGO_0973), asp4 (SGO_0977), and asp5 (SGO_0978); secY2 (SGO_0970), <t>secA2</t> (SGO_0974).
Translocation Machinery, supplied by Seca, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Danaher Inc securityguard column protection
FIGURE 1. Accessory sec locus of S. gordonii DL1. Schematic representation of the 20.5-kb accessory sec locus (derived from the genome sequence of S. gordonii CH1: GenBankTM accession number CP000725.1). Surface protein, hsa (SGO_0966); glycosyltransferases, gly (SGO_0968), nss (SGO_0969), gtfA (SGO_0975), and gtfB (SGO_0976); accessory secretion proteins, asp1 (SGO_0971), asp2 (SGO_0972), asp3 (SGO_0973), asp4 (SGO_0977), and asp5 (SGO_0978); secY2 (SGO_0970), <t>secA2</t> (SGO_0974).
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Image Search Results


FIGURE 1. Accessory sec locus of S. gordonii DL1. Schematic representation of the 20.5-kb accessory sec locus (derived from the genome sequence of S. gordonii CH1: GenBankTM accession number CP000725.1). Surface protein, hsa (SGO_0966); glycosyltransferases, gly (SGO_0968), nss (SGO_0969), gtfA (SGO_0975), and gtfB (SGO_0976); accessory secretion proteins, asp1 (SGO_0971), asp2 (SGO_0972), asp3 (SGO_0973), asp4 (SGO_0977), and asp5 (SGO_0978); secY2 (SGO_0970), secA2 (SGO_0974).

Journal: Journal of Biological Chemistry

Article Title: Composition and Activity of the Non-canonical Gram-positive SecY2 Complex

doi: 10.1074/jbc.m116.729806

Figure Lengend Snippet: FIGURE 1. Accessory sec locus of S. gordonii DL1. Schematic representation of the 20.5-kb accessory sec locus (derived from the genome sequence of S. gordonii CH1: GenBankTM accession number CP000725.1). Surface protein, hsa (SGO_0966); glycosyltransferases, gly (SGO_0968), nss (SGO_0969), gtfA (SGO_0975), and gtfB (SGO_0976); accessory secretion proteins, asp1 (SGO_0971), asp2 (SGO_0972), asp3 (SGO_0973), asp4 (SGO_0977), and asp5 (SGO_0978); secY2 (SGO_0970), secA2 (SGO_0974).

Article Snippet: The core components of the accessory Sec system are SecA2 and SecY2 (homologs of general Sec proteins SecA and SecY, respectively (11)), along with three accessory Sec proteins: Asp1, Asp2, and Asp3 (12).

Techniques: Derivative Assay, Sequencing

FIGURE5.SDS-PAGEanalysisofpurifiedSecA2.CoomassieBlue-stainedgelof purified S. gordonii SecA2 (2 g of protein) with a band between 75 and 100 kDa (the predicted mass is 92 kDa). Protein bands between 37- and 50-kDa markers were presumed to be degradation products due to proteolysis.

Journal: Journal of Biological Chemistry

Article Title: Composition and Activity of the Non-canonical Gram-positive SecY2 Complex

doi: 10.1074/jbc.m116.729806

Figure Lengend Snippet: FIGURE5.SDS-PAGEanalysisofpurifiedSecA2.CoomassieBlue-stainedgelof purified S. gordonii SecA2 (2 g of protein) with a band between 75 and 100 kDa (the predicted mass is 92 kDa). Protein bands between 37- and 50-kDa markers were presumed to be degradation products due to proteolysis.

Article Snippet: The core components of the accessory Sec system are SecA2 and SecY2 (homologs of general Sec proteins SecA and SecY, respectively (11)), along with three accessory Sec proteins: Asp1, Asp2, and Asp3 (12).

Techniques: Purification

FIGURE 6. ATPase activities of S. gordonii SecA2 with and without SecY2- Asp4-Asp5. Steady-state ATPase activity of 0.3 M S. gordonii SecA2 or E. coli SecAinthepresenceof0.46MS. gordoniiSecY2-Asp4-Asp5orE. coliSecYEG, reconstituted in proteoliposomes. Statistical significance is indicated by an asterisk (*, p 0.05, t test). No statistical significance (NS) is also indicated. Error bars are S.E. (n 5).

Journal: Journal of Biological Chemistry

Article Title: Composition and Activity of the Non-canonical Gram-positive SecY2 Complex

doi: 10.1074/jbc.m116.729806

Figure Lengend Snippet: FIGURE 6. ATPase activities of S. gordonii SecA2 with and without SecY2- Asp4-Asp5. Steady-state ATPase activity of 0.3 M S. gordonii SecA2 or E. coli SecAinthepresenceof0.46MS. gordoniiSecY2-Asp4-Asp5orE. coliSecYEG, reconstituted in proteoliposomes. Statistical significance is indicated by an asterisk (*, p 0.05, t test). No statistical significance (NS) is also indicated. Error bars are S.E. (n 5).

Article Snippet: The core components of the accessory Sec system are SecA2 and SecY2 (homologs of general Sec proteins SecA and SecY, respectively (11)), along with three accessory Sec proteins: Asp1, Asp2, and Asp3 (12).

Techniques: Activity Assay

FIGURE 7. Molecular dynamics simulation of SecY2-Asp4-Asp5-SecA2 model. A, homology model of SecY2-Asp4-Asp5-SecA2 was created using Modeler and aligned to the crystal structure of SecYEG-SecA from T. maritima (shown in yellow), Protein Data Bank code 3DIN. Green, SecY2; blue, Asp4; pink, Asp5; beige, SecA2. r.m.s.d. of the alignment of the backbone atoms of the 2 structures is 0.95 Å. B, r.m.s.d. analysis of C- atoms over the course of a 100-ns molecular dynamics simulation. The system stabilized within 10 ns, at an r.m.s.d. of 0.7 nm from its original position. C, pre- and post-simulation images of S. gordonii SecY2-Asp4-Asp5-SecA2 after 100 ns.

Journal: Journal of Biological Chemistry

Article Title: Composition and Activity of the Non-canonical Gram-positive SecY2 Complex

doi: 10.1074/jbc.m116.729806

Figure Lengend Snippet: FIGURE 7. Molecular dynamics simulation of SecY2-Asp4-Asp5-SecA2 model. A, homology model of SecY2-Asp4-Asp5-SecA2 was created using Modeler and aligned to the crystal structure of SecYEG-SecA from T. maritima (shown in yellow), Protein Data Bank code 3DIN. Green, SecY2; blue, Asp4; pink, Asp5; beige, SecA2. r.m.s.d. of the alignment of the backbone atoms of the 2 structures is 0.95 Å. B, r.m.s.d. analysis of C- atoms over the course of a 100-ns molecular dynamics simulation. The system stabilized within 10 ns, at an r.m.s.d. of 0.7 nm from its original position. C, pre- and post-simulation images of S. gordonii SecY2-Asp4-Asp5-SecA2 after 100 ns.

Article Snippet: The core components of the accessory Sec system are SecA2 and SecY2 (homologs of general Sec proteins SecA and SecY, respectively (11)), along with three accessory Sec proteins: Asp1, Asp2, and Asp3 (12).

Techniques:

FIGURE 8. WGA dot blot analysis of S. gordonii WT and mutants. 2-Fold dilutions of intact cells of S. gordonii WT, secA2, hsa, and asp4 were dot blotted with WGA, which has primary sugar specificity to N-acetylgluco- samine, a major component of Hsa.

Journal: Journal of Biological Chemistry

Article Title: Composition and Activity of the Non-canonical Gram-positive SecY2 Complex

doi: 10.1074/jbc.m116.729806

Figure Lengend Snippet: FIGURE 8. WGA dot blot analysis of S. gordonii WT and mutants. 2-Fold dilutions of intact cells of S. gordonii WT, secA2, hsa, and asp4 were dot blotted with WGA, which has primary sugar specificity to N-acetylgluco- samine, a major component of Hsa.

Article Snippet: The core components of the accessory Sec system are SecA2 and SecY2 (homologs of general Sec proteins SecA and SecY, respectively (11)), along with three accessory Sec proteins: Asp1, Asp2, and Asp3 (12).

Techniques: Dot Blot

FIGURE 12. Model of SecA2 bound to SecY2-Asp4-Asp5 translocon in S. gordonii. SecY2, Asp4, and Asp5 form the cytoplasmic membrane translo- con of the accessory Sec system, where SecA2 associates with the complex working as an ATPase to provide energy for Hsa substrate translocation.

Journal: Journal of Biological Chemistry

Article Title: Composition and Activity of the Non-canonical Gram-positive SecY2 Complex

doi: 10.1074/jbc.m116.729806

Figure Lengend Snippet: FIGURE 12. Model of SecA2 bound to SecY2-Asp4-Asp5 translocon in S. gordonii. SecY2, Asp4, and Asp5 form the cytoplasmic membrane translo- con of the accessory Sec system, where SecA2 associates with the complex working as an ATPase to provide energy for Hsa substrate translocation.

Article Snippet: The core components of the accessory Sec system are SecA2 and SecY2 (homologs of general Sec proteins SecA and SecY, respectively (11)), along with three accessory Sec proteins: Asp1, Asp2, and Asp3 (12).

Techniques: Membrane, Translocation Assay