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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: Composition and Activity of the Non-canonical Gram-positive SecY2 Complex
doi: 10.1074/jbc.m116.729806
Figure Lengend Snippet: FIGURE 1. Accessory sec locus of S. gordonii DL1. Schematic representation of the 20.5-kb accessory sec locus (derived from the genome sequence of S. gordonii CH1: GenBankTM accession number CP000725.1). Surface protein, hsa (SGO_0966); glycosyltransferases, gly (SGO_0968), nss (SGO_0969), gtfA (SGO_0975), and gtfB (SGO_0976); accessory secretion proteins, asp1 (SGO_0971), asp2 (SGO_0972), asp3 (SGO_0973), asp4 (SGO_0977), and asp5 (SGO_0978); secY2 (SGO_0970), secA2 (SGO_0974).
Article Snippet: The core components of the accessory Sec system are
Techniques: Derivative Assay, Sequencing
Journal: Journal of Biological Chemistry
Article Title: Composition and Activity of the Non-canonical Gram-positive SecY2 Complex
doi: 10.1074/jbc.m116.729806
Figure Lengend Snippet: FIGURE5.SDS-PAGEanalysisofpurifiedSecA2.CoomassieBlue-stainedgelof purified S. gordonii SecA2 (2 g of protein) with a band between 75 and 100 kDa (the predicted mass is 92 kDa). Protein bands between 37- and 50-kDa markers were presumed to be degradation products due to proteolysis.
Article Snippet: The core components of the accessory Sec system are
Techniques: Purification
Journal: Journal of Biological Chemistry
Article Title: Composition and Activity of the Non-canonical Gram-positive SecY2 Complex
doi: 10.1074/jbc.m116.729806
Figure Lengend Snippet: FIGURE 6. ATPase activities of S. gordonii SecA2 with and without SecY2- Asp4-Asp5. Steady-state ATPase activity of 0.3 M S. gordonii SecA2 or E. coli SecAinthepresenceof0.46MS. gordoniiSecY2-Asp4-Asp5orE. coliSecYEG, reconstituted in proteoliposomes. Statistical significance is indicated by an asterisk (*, p 0.05, t test). No statistical significance (NS) is also indicated. Error bars are S.E. (n 5).
Article Snippet: The core components of the accessory Sec system are
Techniques: Activity Assay
Journal: Journal of Biological Chemistry
Article Title: Composition and Activity of the Non-canonical Gram-positive SecY2 Complex
doi: 10.1074/jbc.m116.729806
Figure Lengend Snippet: FIGURE 7. Molecular dynamics simulation of SecY2-Asp4-Asp5-SecA2 model. A, homology model of SecY2-Asp4-Asp5-SecA2 was created using Modeler and aligned to the crystal structure of SecYEG-SecA from T. maritima (shown in yellow), Protein Data Bank code 3DIN. Green, SecY2; blue, Asp4; pink, Asp5; beige, SecA2. r.m.s.d. of the alignment of the backbone atoms of the 2 structures is 0.95 Å. B, r.m.s.d. analysis of C- atoms over the course of a 100-ns molecular dynamics simulation. The system stabilized within 10 ns, at an r.m.s.d. of 0.7 nm from its original position. C, pre- and post-simulation images of S. gordonii SecY2-Asp4-Asp5-SecA2 after 100 ns.
Article Snippet: The core components of the accessory Sec system are
Techniques:
Journal: Journal of Biological Chemistry
Article Title: Composition and Activity of the Non-canonical Gram-positive SecY2 Complex
doi: 10.1074/jbc.m116.729806
Figure Lengend Snippet: FIGURE 8. WGA dot blot analysis of S. gordonii WT and mutants. 2-Fold dilutions of intact cells of S. gordonii WT, secA2, hsa, and asp4 were dot blotted with WGA, which has primary sugar specificity to N-acetylgluco- samine, a major component of Hsa.
Article Snippet: The core components of the accessory Sec system are
Techniques: Dot Blot
Journal: Journal of Biological Chemistry
Article Title: Composition and Activity of the Non-canonical Gram-positive SecY2 Complex
doi: 10.1074/jbc.m116.729806
Figure Lengend Snippet: FIGURE 12. Model of SecA2 bound to SecY2-Asp4-Asp5 translocon in S. gordonii. SecY2, Asp4, and Asp5 form the cytoplasmic membrane translo- con of the accessory Sec system, where SecA2 associates with the complex working as an ATPase to provide energy for Hsa substrate translocation.
Article Snippet: The core components of the accessory Sec system are
Techniques: Membrane, Translocation Assay